L-NAME (hydrochloride)

51298-62-5

C7H16ClN5O4

H2O : >= 32 mg/mL (118.65 mM); DMSO : 100 mg/mL (370.80 mM; Need ultrasonic)

Immunology/Inflammation

L-NAME hydrochloride inhibits NOS with an IC₅₀ of 70 uM. L-NAME is a precursor to NOS inhibitor L-NOARG which has an IC₅₀ value of 1.4 uM. IC50 & Target: IC50: 70 uM (NOS)^[1] In Vitro: L-arginine analogues are widely used inhibitors of nitric oxide synthase (NOS) activity, with N^w-nitro-L-arginine methyl ester (L-NAME) being at the head^[2]. Freshly dissolved L-NAME is a 50 fold less potent inhibitor of purified brain NOS (mean IC₅₀= 70 uM) than L-NOARG (IC₅₀= 1.4 uM), but the apparent inhibitory potency of L-NAME approached that of L-NOARG upon prolonged incubation at neutral or alkaline pH. HPLC analyses reveal that NOS inhibition by L-NAME closely correlated with hydrolysis of the drug to L-NOARG^[1]. In Vivo: L-NAME infusion significantly decreases NKT-leukocyte level, tumor-necrosis factor (TNF)-alpha production by T-splenocytes and macrophages, and interferon-gamma production by T-leukocytes, monocytes, and T-splenocytes, as well as increased interleukin-6 production by T-leukocytes and monocytes and nitrate/nitrite production by T-leukocytes^[3]. There is increasing evidence that nitric oxide may be involved in learning and memory. l-NAME produces a task-dependent impairment of fear extinction, and implies that nitric oxide signaling is involved in memory process of certain fear extinction tasks^[4]. Chronic L-NAME administration induces cardiac hypertrophy in rodent models. Six weeks L-NAME administration induces significant cardiac hypertrophy compared to control hearts^[5].