Sarsasapogenin

126-19-2

C27H44O3

Ethanol : 1 mg/mL (2.40 mM; Need ultrasonic)

NF-kappaB

Sarsasapogenin is a sapogenin from the Chinese medical herb Anemarrhena asphodeloides Bunge, with antidiabetic, anti-oxidative, anticancer and anti-inflamatory activities. In Vitro: Sarsasapogenin (20-80 uM) induces apoptosis via caspase-dependent mitochondrial pathway in heLa cells. Sarsasapogenin also induces apoptosis via the caspase-dependent mitochondrial apoptotic pathway. Sarsasapogenin (60 uM)-induced ROS generation triggers ER stress and mitochondrial dysfunction^[1]. Sarsasapogenin potently inhibits NF-kappaB and MAPK activation, as well as IRAK1, TAK1, and IkappaBalpha phosphorylation in LPS-stimulated macrophages. Moreover, Sarsasapogenin inhibits the binding of LPS to macrophage Toll-like receptor 4, and polarization of M2 to M1 macrophages^[3]. In Vivo: Sarsasapogenin (20 and 40 mg/kg) significantly restores the sucrose preference deficit induced by olfactory bulbectomy (OB), and increases locomotor activity. Sarsasapogenin groups (20 and 40 mg/kg) have significantly lower immobility times, higher AChE protein expression levels than the OB group. Furthermore, Sarsasapogenin (20 and 40 mg/kg) groups have significantly higher alpha7-nAChR protein expression, and increases higher alpha4-nAChR protein expression levels compared to rats in the OB group^[2]. Sarsasapogenin (5 or 10 mg/kg, p.o.) inhibits TNBS-induced colon shortening and myeloperoxidase activity in mice, reducing NF-kappaB activation and interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha, and IL-6 levels, while simultaneously increasing IL-10^[3].