NCI-H358 Cells

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Profit Organisation/Nutzung: CLS_CLEAR.pdf
Non-Profit Organisation/Nutzung: Non_Profit_CLS_Supply_Agreement.pdf

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ArtNr	CLS-300430
Hersteller	CLS Cell Lines Service
Menge	1 cryovial
Kategorie	Cell Lines & Cell Culture Cell Lines
Typ	Cell line
Zertifikat	For a certificate of analysis, please send an inquiry to info@hoelzel.de.
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	NCI-H358,H-358,NCIH358
Lieferbar
Manufacturer - Category	Lung cancer cell lines
Description	NCI-H358, also known as H-358 or NCIH358, is an epithelial-like cell line derived from a patient with bronchioalveolar carcinoma, a subtype of non-small cell lung cancer (NSCLC). These cells display ultrastructural characteristics typical of Clara cells, such as specific cytoplasmic features. NCI-H358 cells are particularly relevant in cancer research focused on NSCLC, especially for exploring the biology and treatment of lung adenocarcinomas. This cell line is crucial for studying the effectiveness of therapies targeting the Epidermal Growth Factor Receptor (EGFR), as mutations in EGFR are a significant focus in the treatment of NSCLC. Additionally, NCI-H358 cells are valuable for investigating the role of KRAS mutations, which are prevalent in lung cancer and known to drive oncogenic activity. The study of these mutations in NCI-H358 cells helps to elucidate the molecular pathways involved in lung cancer progression and resistance to therapies. The NCI-H358 cell line harbors a homozygous deletion of p53, a major tumor suppressor. The H358 lung cancer cell line is also used to assess the potential of novel therapeutic approaches, such as SOS1 PROTACs, aimed at targeting specific oncogenic pathways. In summary, the NCI-H358 cell line, derived from bronchioalveolar carcinoma, is a vital tool in NSCLC research. It is instrumental for studying EGFR-targeted therapies and KRAS mutations' role in lung cancer. Its application in cancer research extends to the development of new therapeutic strategies aimed at mitigating the effects of oncogenic mutations and improving patient outcomes in lung cancer.
Tissue	Lung
Growth properties	Adherent
Cell type	Club cell
Disease	Minimally invasive lung adenocarcinoma
Age	Age unspecified
Gender	Male
Ethnicity	European
Biosafety Level	1
Culture Medium	RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Medium Supplements	Supplement the medium with 10% FBS
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Freeze Medium	CM-1 (Cytion catalog number 800100)
Handling of Cryopreserved Cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions	When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer	Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty	We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Amelogenin	X, Y
Tumorigenic	Yes, in nude mice.
Mutational Profile	p53 homozygously deleted
Protein Expression	UGT -, GST +, PST +, p53 -

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Menge: 1 cryovial

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