Recombinant Human Interleukin 12 (a. a 503) Polypeptide

The lyophilized product, though stable, for 3 weeks at room temperature, is best stored at –20°C. After reconstitution, short term (up to 1 week) store at 2-8oC. Long term, add 0.1% HSA or BSA, aliquot and store at –20°C. Avoid multiple freeze/thaw cycles.

Recombinant, Human IL-12 is a glycosylated disulfide linked heterodimeric polypeptide, containing 503 amino acids and having a molecular weight of 75kDa comprised, of disulfide-bonded 35kDa (p35) and 40kDa (p40) subunits. The sequence of the, first five N-terminal amino acids was determined to be Arg-Asn-Leu-Pro-Val at, the p35 subunit and Ile-Trp-Glu-Leu-Lys at the p40 subunit. Contains less, than 1% dimers and aggregates.

Purified, Lyophilized, Reconstitute using sterile deionized, water to a concentration >100ug/ml. Further dilutions can be made in other aqueous buffers.

1mg/ml (OD280nm), (prior to lyophilization)

IL12A interleukin 12A (naturalkiller cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) [ Homo sapiens ]

NP_000873

African, trypanosomiasis, Allograft rejection, Amoebiasis, Chagas disease (American, trypanosomiasis), Cytokine-cytokine receptor interaction, Herpes simplex, infection, IL12-mediated signaling events, IL27-mediated signaling events, Influenza, A, Jak-STAT signaling pathway, Leishmaniasis, Malaria, Pertussis, RIG-I-like, receptor signaling pathway, RIG-I-like receptor signaling pathway

Interleukin-12, subunit alpha, is a protein that in humans is encoded by the IL12A gene. This gene encodes a, subunit of a cytokine that acts on T and natural killer cells, and has a, broad array of biological activities. The cytokine is a disulfide-linked, heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD, subunit that is a member of the cytokine receptor family. This cytokine is, required for the T-cell-independent induction of interferon (IFN)-gamma, and, is important for the differentiation of both Th1 and Th2 cells. The responses, of lymphocytes to this cytokine are mediated by the activator of, transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found, to be required for the signaling process of this cytokine in innate immunity.