Recombinant Human Chemokine (C-X-C Motif) Ligand 10 Polypeptide (a. a. 77)

The lyophilized product, though stable for 3 weeks at roomtemperature, is best stored at –20°C. After reconstitution, short term (up to1 week) store at 2-8oC. Long term, add 0.1% HSA or BSA, aliquot and store at–20°C. Avoid multiple freeze/thaw cycles.

Recombinant Human CCL19 is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular weight of8, 809 Da, was expressed in E. coli. The sequence of the first five N-terminalamino acids was determined to be Gly-Thr-Asn-Asp-Ala. Contains less than 1%dimers and aggregates.

Purified, Lyophilized Reconstitute using sterile deionized waterto a concentration >100ug/ml. Further dilutions can be made inother aqueous buffers.

Lotspecific (OD280nm) (prior to lyophilization)

CCL19chemokine (C-C motif) ligand 19 [ Homo sapiens ]

NP_006265

Chemokinereceptors bind chemokines, Chemokine signaling pathway, Class A/1(Rhodopsin-like receptors), Cytokine-cytokine receptor interaction

MIP3chemokines are among many novel beta chemokines recently identified throughbioinformatics in the Expressed Sequence Tag (EST) database. They aredistantly related to other beta chemokines (20-30% aa sequence homology).MIP3 chemokines are useful probes for studying the biology of inflammationand cell migration. Their expression is strongly up-regulated by inflammatorysignals and down-regulated by the anti-inflammatory cytokine IL10. MIP3 betais also known as ELC (EBI1-1 ligand chemokine) and is constitutivelyexpressed in various lymphoid tissues (thymus, lymph nodes, appendix andspleen). MIP3 beta is a chemoattractant for cultured human lymphocytes, dendritic cells, human T lymphoblastoid CEM NKR2 and hemopoietic progenitorcells. It may be involved in G Protein activation in human NK cells. MIP3 betais a unique functional ligand for CCR7. The MIP3 beta gene has been mapped tochormosome 9p13.