Histone H2AX Antibody

Polyclonal Antibody

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Synthesized peptide derived from internal of human Histone H2AX.

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

The MGC Project Team, Genome Res. 14:2121-2127(2004).
Rogakou E.P., J. Biol. Chem. 273:5858-5868(1998).
Rogakou E.P., J. Biol. Chem. 275:9390-9395(2000).

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Epigenetics and Nuclear Signaling

Synthesized peptide derived from internal of Human Histone H2AX.