CCNC (Ab-275) Antibody

ELISA, WB; WB:1:500-1:3000

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Synthesized non-phosphopeptide derived from human Cyclin C around the phosphorylation site of serine 275 (N-G-S(p)-Q-N).

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.

Lew D.J., Cell 66:1197-1206(1991).
Li H., Genomics 32:253-259(1996).
Mungall A.J., Nature 425:805-811(2003).

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Cell Biology

Synthesized non-phosphopeptide derived from Human Cyclin C around the phosphorylation site of serine 275 (N-G-S(p)-Q-N).