Phospho-KCNJ11 (Thr224) Antibody

ELISA, WB, IF; WB:1:500-1:1000, IF:1:100-1:200

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Peptide sequence around phosphorylation site of threonine 224 (K-T-TP-S-P) derived from Human Kir6.2.

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium, as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium By similarity. Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Neuroscience

Peptide sequence around phosphorylation site of threonine 224 (K-T-TP-S-P) derived from Human Kir6.2.