Phospho-RB1 (Thr826) Antibody

Polyclonal Antibody

RB1

Exon 17 tumor GOS561 substitution mutation causes premature stop antibody, GOS563 exon 17 substitution mutation causes premature stop antibody, OSRC antibody, Osteosarcoma antibody, p105-Rb antibody, P105RB antibody, PP105 antibody, pp110 antibody, PPP1R130 antibody, pRb antibody, Prepro retinoblastoma associated protein antibody, Protein phosphatase 1 regulatory subunit 130 antibody, Rb antibody, RB transcriptional corepressor 1 antibody, RB_HUMAN antibody, RB1 antibody, RB1 gene antibody, Retinoblastoma 1 antibody, Retinoblastoma suspectibility protein antibody, Retinoblastoma-associated protein antibody

Peptide sequence around phosphorylation site of threonine 826 (K-M-T(p)-P-R) derived from Human Retinoblastoma.

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex By similarity. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Cell Biology

Peptide sequence around phosphorylation site of threonine 826 (K-M-T(p)-P-R) derived from Human Retinoblastoma.