Recombinant Human Phosphoenolpyruvate carboxykinase, cytosolic [GTP](PCK1)

Metabolism

Not Test

Homo sapiens (Human)

MPPQLQNGLNLSAKVVQGSLDSLPQAVREFLENNA ELCQPDHIHICDGSEEENGRLLGQMEEEGILRRLK KYDNCWLALTDPRDVARIESKTVIVTQEQRDTVPI PKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMY VIPFSMGPLGSPLSKIGIELTDSPYVVASMRIMTR MGTPVLEAVGDGEFVKCLHSVGCPLPLQKPLVNNW PCNPELTLIAHLPDRREIISFGSGYGGNSLLGKKC FALRMASRLAKEEGWLAEHMLILGITNPEGEKKYL AAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWM KFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKT IQKNTIFTNVAETSDGGVYWEGIDEPLASGVTITS WKNKEWSSEDGEPCAHPNSRFCTPASQCPIIDAAW ESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVF VGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNF GKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKF LWPGFGENSRVLEWMFNRIDGKASTKLTPIGYIPK EDALNLKGLGHINMMELFSISKEFWEKEVEDIEKY LEDQVNADLPCEIEREILALKQRISQM

1-622aa

73.2 kDa

The DNA sequence and comparative analysis of human chromosome 20.Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.Nature 414:865-871(2001)

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Cytoplasm

Major sites of expression are liver, kidney and adipocytes.

N-terminal 6xHis-tagged