Recombinant Human Ribonucleoside-diphosphate reductase large subunit(RRM1)

Epigenetics and Nuclear Signaling

RRM1

MHVIKRDGRQERVMFDKITSRIQKLCYGLNMDFVD PAQITMKVIQGLYSGVTTVELDTLAAETAATLTTK HPDYAILAARIAVSNLHKETKKVFSDVMEDLYNYI NPHNGKHSPMVAKSTLDIVLANKDRLNSAIIYDRD FSYNYFGFKTLERSYLLKINGKVAERPQHMLMRVS VGIHKEDIDAAIETYNLLSERWFTHASPTLFNAGT NRPQLSSCFLLSMKDDSIEGIYDTLKQCALISKSA GGIGVAVSCIRATGSYIAGTNGNSNGLVPMLRVYN NTARYVDQGGNKRPGAFAIYLEPWHLDIFEFLDLK KNTGKEEQRARDLFFALWIPDLFMKRVETNQDWSL MCPNECPGLDEVWGEEFEKLYASYEKQGRVRKVVK AQQLWYAIIESQTETGTPYMLYKDSCNRKSNQQNL GTIKCSNLCTEIVEYTSKDEVAVCNLASLALNMYV TSEHTYDFKKLAEVTKVVVRNLNKIIDINYYPVPE ACLSNKRHRPIGIGVQGLADAFILMRYPFESAEAQ LLNKQIFETIYYGALEASCDLAKEQGPYETYEGSP VSKGILQYDMWNVTPTDLWDWKVLKEKIAKYGIRN SLLIAPMPTASTAQILGNNESIEPYTSNIYTRRVL SGEFQIVNPHLLKDLTERGLWHEEMKNQIIACNGS IQSIPEIPDDLKQLYKTVWEISQKTVLKMAAERGA FIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTG MYYLRTRPAANPIQFTLNKEKLKDKEKVSKEEEEK ERNTAAMVCSLENRDECLMCGS

Full Length

N-terminal 6xHis-SUMO-tagged

Ribonucleoside-diphosphate reductase subunit M1Ribonucleotide reductase large subunit

Structural basis for allosteric regulation of human ribonucleotide reductase by nucleotide-induced oligomerization.Fairman J.W., Wijerathna S.R., Ahmad M.F., Xu H., Nakano R., Jha S., Prendergast J., Welin R.M., Flodin S., Roos A., Nordlund P., Li Z., Walz T., Dealwis C.G.Nat. Struct. Mol. Biol. 18:316-322(2011)

Liquid or Lyophilized powder

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.

Cytoplasm

N-terminal 6xHis-SUMO-tagged