Hersteller |
Cusabio
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
Human |
Format |
Liquid or Lyophilized powder |
Menge |
100ug |
Host |
E.coli |
ArtNr |
CSB-EP006463HU-100 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Research Areas |
Stem Cells |
Target / Protein |
CYR61 |
Biologically Active |
Not Test |
Expression System |
E.coli |
Species of origin |
Homo sapiens (Human) |
Uniprot ID |
O00622 |
AA Sequence |
TCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQ LNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQ SEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVG CIPLCPQELSLPNLGCPNPRLVKVTGQCCEEWVCD EDSIKDPMEDQDGLLGKELGFDASEVELTRNNELI AVGKGSSLKRLPVFGMEPRILYNPLQGQKCIVQTT SWSQCSKTCGTGISTRVTNDNPECRLVKETRICEV RPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCL SVKKYRPKYCGSCVDGRCCTPQLTRTVKMRFRCED GETFSKNVMMIQSCKCNYNCPHANEAAFPFYRLFN DIHKFRD |
Tag Info |
N-terminal 6xHis-SUMO-tagged |
Expression Region |
25-381aa |
Protein Length |
Full Length of Mature Protein |
MW |
55.4 kDa |
Distributor Discount |
50% off the list price |
Alternative Name(s) |
CCN family member 1 Cysteine-rich angiogenic inducer 61 Insulin-like growth factor-binding protein 10 Short name: IBP-10 Short name: IGF-binding protein 10 Short name: IGFBP-10 Protein GIG1 |
Relevance |
Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. |
Reference |
The angiogenic factor Cyr61 activates a genetic program for wound healing in human skin fibroblasts.Chen C.-C., Mo F.-E., Lau L.F.J. Biol. Chem. 276:47329-47337(2001) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. |
Subcellular Location |
Secreted |
Protein Families |
CCN family |
Tag Information |
N-terminal 6xHis-SUMO-tagged |
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