Hersteller |
Cusabio
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
Format |
Liquid or Lyophilized powder |
Menge |
100ug |
Host |
E.coli |
ArtNr |
CSB-EP019652HU-100 |
Konjugat/Tag |
GST |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Research Areas |
Signal Transduction |
Uniprot ID |
O76081 |
Gene Names |
RGS20 |
Organism |
Homo sapiens (Human) |
AA Sequence |
EPAGASSPAGRVDGGLQMGSERMEMRKRQMPAAQD TPGAAPGQPGAGSRGSNACCFCWCCCCSCSCLTVR NQEDQRPTIASHELRADLPTWEESPAPTLEEVNAW AQSFDKLMVTPAGRNAFREFLRTEFSEENMLFWMA CEELKKEANKNIIEEKARIIYEDYISILSPKEVSL DSRVREVINRNMVEPSQHIFDDAQLQIYTLMHRDS YPRFMNSAVYKDLLQSLSEKSIEA |
Expression Region |
1-234aa |
Sequence Info |
Full Length of BC015614 |
Source |
E.coli |
Tag Info |
N-terminal GST-tagged |
MW |
53.4 kDa |
Alternative Name(s) |
Gz-selective GTPase-activating protein |
Relevance |
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins |
Reference |
RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz GTPase-activating protein subfamily. Wang J., Ducret A., Tu Y., Kozasa T., Aebersold R., Ross E.M. J. Biol. Chem. 273:26014-26025(1998) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins (By similarity). |
Subcellular Location |
Membrane, Lipid-anchor, Nucleus, Cytoplasm |
Tissue Specificity |
Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe. |
Tag Information |
N-terminal GST-tagged |
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