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Recombinant Raphanus sativus Defensin-like protein 2(AFP2)

Hersteller Cusabio
Kategorie
Typ Proteins Recombinant
Specific against other
Format Liquid or Lyophilized powder
Menge 1mg
Host E.coli
ArtNr CSB-EP339081RJP-1
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Research Areas
Others
Target / Protein
AFP2
Biologically Active
Not Test
Expression System
E.coli
Species of origin
Raphanus sativus (Radish)
Uniprot ID
P30230
AA Sequence
QKLCQRPSGTWSGVCGNNNACKNQCIRLEKARHGS CNYVFPAHKCICYFPC
Tag Info
N-terminal 6xHis-SUMO-tagged
Expression Region
30-80aa
Protein Length
Full Length of Mature Protein
MW
21.7 kDa
Distributor Discount
50% off the list price
Alternative Name(s)
Cysteine-rich antifungal protein 2
Short name:
AFP2
Short name:
RAFP2
Relevance
Possesses antifungal activity sensitive to inorganic cations. Induces potential changes in fungal membranes and increased K+ efflux and Ca2+ uptake.
Reference
Mutational analysis of a plant defensin from radish (Raphanus sativus L.) reveals two adjacent sites important for antifungal activity.
De Samblanx G.W., Goderis I.J., Thevissen K., Raemaekers R., Fant F., Borremans F., Acland D.P., Osborn R.W., Patel S., Broekaert W.F.
J. Biol. Chem. 272:1171-1179(1997).
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Possesses antifungal activity sensitive to inorganic cations. Induces potential changes in fungal membranes and increased K(+) efflux and Ca(2+) uptake.
Subcellular Location
Secreted
Protein Families
DEFL family
Tag Information
N-terminal 6xHis-SUMO-tagged

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1mg
Lieferbar: In stock
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