Hersteller |
Cusabio
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
Human |
Format |
Liquid or Lyophilized powder |
Menge |
10ug |
Host |
E.coli |
ArtNr |
CSB-EP745333HU-10 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Research Areas |
Others |
Target / Protein |
EIF3M |
Biologically Active |
Not Test |
Expression System |
E.coli |
Species of origin |
Homo sapiens (Human) |
Uniprot ID |
Q7L2H7 |
AA Sequence |
SVPAFIDISEEDQAAELRAYLKSKGAEISEENSEG GLHVDLAQIIEACDVCLKEDDKDVESVMNSVVSLL LILEPDKQEALIESLCEKLVKFREGERPSLRLQLL SNLFHGMDKNTPVRYTVYCSLIKVAASCGAIQYIP TELDQVRKWISDWNLTTEKKHTLLRLLYEALVDCK KSDAASKVMVELLGSYTEDNASQARVDAHRCIVRA LKDPNAFLFDHLLTLKPVKFLEGELIHDLLTIFVS AKLASYVKFYQNNKDFIDSLGLLHEQNMAKMRLLT FMGMAVENKEISFDTMQQELQIGADDVEAFVIDAV RTKMVYCKIDQTQRKVVVSHSTHRTFGKQQWQQLY DTLNAWKQNLNKVKNSLLSLSDT |
Tag Info |
N-terminal 6xHis-SUMO-tagged |
Expression Region |
2-374aa |
Protein Length |
Full Length of Mature Protein |
MW |
58.4 kDa |
Distributor Discount |
50% off the list price |
Alternative Name(s) |
Short name: eIF3m Alternative name(s): Fetal lung protein B5 Short name: hFL-B5 PCI domain-containing protein 1 |
Relevance |
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2). |
Reference |
A new class of receptor for herpes simplex virus has heptad repeat motifs that are common to membrane fusion proteins.Perez A., Li Q.-X., Perez-Romero P., DeLassus G., Lopez S.R., Sutter S., McLaren N., Fuller A.O.J. Virol. 79:7419-7430(2005). |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis |
Subcellular Location |
Cytoplasm |
Protein Families |
EIF-3 subunit M family |
Tissue Specificity |
Broadly expressed. |
Tag Information |
N-terminal 6xHis-SUMO-tagged |
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