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Recombinant Bertholletia excelsa 2S sulfur-rich seed storage protein 1(BE2S1),partial

Hersteller Cusabio
Kategorie
Typ Proteins Recombinant
Specific against other
Format Liquid or Lyophilized powder
Menge 100ug
Host Yeast
ArtNr CSB-YP356250BGJ-100
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Research Areas
Allergen
Uniprot ID
P04403
Gene Names
BE2S1
Organism
Brazil nut
AA Sequence
QEECREQMQRQQMLSHCRMYMRQQMEESPYQTMPR RGMEPHMSECCEQLEGMDESCRCEGLRMMMMRMQQ EEMQPRGEQMRRMMRLAENIPSRCNLSPMRCPMGG
Expression Region
37-142aa
Sequence Info
Partial
Source
Yeast
Tag Info
N-terminal 6xHis-tagged
MW
14.8 kDa
Alternative Name(s)
Alternative name(s):
Allergen: Ber e 1
Cleaved into the following 2 chains:
2S sulfur-rich seed storage protein small chain 1
Alternative name(s):
2S albumin 1 small subunit
2S sulfur-rich seed storage protein large chain 1B
Alternative name(s):
2S albumin 1 large subunit
Relevance
This is a 2S seed storage protein.
Reference
The disulphide mapping, folding and characterisation of recombinant Ber e 1, an allergenic protein, and SFA8, two sulphur-rich 2S plant albumins.Alcocer M.J., Murtagh G.J., Bailey K., Dumoulin M., Meseguer A.S., Parker M.J., Archer D.B.J. Mol. Biol. 324:165-175(2002).
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
This is a 2S seed storage protein.
Protein Families
2S seed storage albumins family
Tag Information
N-terminal 6xHis-tagged

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100ug
Lieferbar: In stock
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