Control for Immunoprecipitation IgG beads

As part of the Signal-Seeker product line, IgG control beads (CIG01) have been developed as highly specific negative control beads for Cytoskeleton's mouse, monoclonal antibody-based affinity bead products. These control beads are far superior to beads alone, because it is a better representative of the actual IP condition. The IgG antibodies are covalently attached to minimize antibody heavy and light chain leaching, which makes interpretation of results easier. Additionally, these control beads are effective for pre-clearing lysates without risk of contamination with IgG antibody. IgG control beads (CIG01) are included in our comprehensive Signal-Seeker antibody based Detection Kits and utilizing these kits are recommend for first time PTM investigators.

Each lot of affinity-bead is quality controlled to provide high batch to batch consistency, see COA documents.

Application 1: Utilization of IgG control beads

IgG control beads are compatible with both the phosphotryosine and SUMOylation 2/3 affinity beads and Signal-Seeker kits. Note these beads do not have cross reactivity with either of these PTMs (Figure 1).

Figure 1: A431 cell were lysed with BlastR buffer. 1mg of protein was incubated with APY03-beads (pY) or CIG01 (IgG control). Samples were separated by SDS-PAGE and transferred to PVDF. Western blot using a phosphotyrosine antibody (APY03) was performed. 1mg of protein was incubated with ASM24-beads (SUM0 2/3) or CIG01 (IgG control).Samples were separated by SDS-PAGE and transferred to PVDF. Western blot using a SUMO 2/3 antibody (ASM23) was performed.

Amount:
Each package contains enough IgG control beads for 10 reactions.

For more information contact: signalseekercytoskeleton.com

Associated Products:

Signal-Seeker Phosphotyrosine Detection Kit (Cat. # BK160)

Signal-Seeker SUMOylation 2/3 Detection Kit (Cat. # BK162)

Signal-Seeker Phosphotyrosine Affinity Beads (Cat.# APY03-beads)

Signal-Seeker SUMOylation 2/3 Affinity Beads (Cat.# ASM24-beads)

Signal-Seeker: BlastR Rapid Lysate Prep Kit (Cat. # BLR01)