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Arp2/3 Protein Complex (Bovine brain)

Hersteller Cytoskeleton
Kategorie
Typ Proteins
Specific against Bovine
Menge 2 x 50 ug
ArtNr RP01-A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Delivery Time
1-2 Weeks
Shipping Temp.
AT
Storage on Arrival
4C
faqs

Question 1:  What is the best way to store Arp2/3 (Cat. # RP01)?

Answer 1:  Store the lyophilized Arp2/3 protein desiccated (<, 10% humidity) at 4°, C where it is stable for 6 months.  Lyophilized protein can also be stored desiccated at -70°, C where it will be stable for 6 months.  However, at -70°, C the rubber seal in the lid of the tube could crack and allow in moisture.  Therefore we recommend storing at 4°, C.  If stored at -70°, C, it is imperative to include desiccant with the lyophilized protein if this storage condition is utilized.    Resuspend the protein complex to 5 mg/ml with 10 &mu, l of cold Milli-Q water.  When resuspended, the complex is in the following buffer: 20 mM Tris pH 7.5, 25 mM KCl, 1 mM MgCl2, 0.5 mM EDTA, 0.1 mM ATP, 1.0% (v/v) dextran and 5% (v/v) sucrose.  The protein should then be aliquoted into experiment-sized amounts, snap frozen in liquid nitrogen and stored at -70°, C where it is stable for 6 months.  Further dilution of Arp2/3 should be made in the following buffer: 20 mM Tris pH 7.5, 25 mM KCl, 1 mM MgCl2 and 1 mM DTT (Note: add DTT to the buffer immediately prior to use).  NOTE: It is very important to snap freeze the Arp2/3 protein in liquid nitrogen as other methods of freezing will result in significantly reduced activity.  Defrost rapidly by placing in a room temperature water bath for 1 min.  Avoid repeated freeze/thaw cycles.

 

Question 2:  Is the Arp2/3 (Cat. # RP01) compatible with the actin polymerization kit (Cat. # BK003)?

Answer 2:  Yes, Arp2/3 works very well with the actin polyermization kit (Cat. # BK003) to study the effects of this actin binding protein on actin polymerization.  Please see the Arp2/3 datasheet (Cat. # RP01) for additional information.  The Arp2/3 complex is able to induce the branched polymerization of actin filaments in vitro at a molar ratio of 1:200 (Arp2/3:actin).  This stimulation is observable in an in vitro polymerization assay, however, the stimulation from Arp2/3 alone is very low under typical polymerization conditions.  In the presence of N-WASP protein (or the VCA domain of N-WASP, Cat. # VCG03), the nucleating activity of Arp2/3 is greatly enhanced.  In the polymerization assay described in the Arp2/3 datasheet, pyrene actin (Cat. # AP05 or BK003) is present at a final concentration of 0.8 &mu, M, the Arp2/3 complex is at 10 nM and the VCA domain (Cat. # VCG03) is at 400 nM.

 

If you have any questions concerning this product, please contact our Technical Service department at infohoelzel.de.

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 2 x 50 ug
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 18.04.2024 

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