Hela Cancer Cell Tubulin Biotinylated

Question 1: Can biotin-labeled HeLa tubulin polymerize just as well as unlabeled HeLa tubulin?

Answer 1: Yes, the biotin-labeled HeLa cell tubulin (Cat. #H003) polymerizes in a similar manner to unlabeled HeLa cell tubulin ((Cat. #H001). In QC testing, the biological activity (polymerization) of biotin HeLa tubulin has been tested as follows: 250 ug of biotin HeLa tubulin are reconstituted to 2 mg/ml in G-PEM buffer pH 6.9 plus 20% v/v glycerol and incubated at 35°C in a cuvette. The optical density at 340 nm is taken at time intervals. A value of 0.075 OD unit indicates that >, 90% of the tubulin has polymerized.

Question 2: What are some specific uses for biotin-labeled HeLa tubulin?

Answer 2: Cytoskeleton&rsquo, s biotin-labeled HeLa cell tubulin (Cat. #H003) can be used 1) to screen for cancer tubulin specific ligands, 2) for microinjections into living cells to track tubulin distribution, 3) immobilizing tubulin onto a solid surface via streptavidin, and 4) with SPA technology (Amersham, Inc.) for drug screening.