Human Gamma-Aminobutyric Acid A Receptor Alpha 2 (gABRa2) ELISA Kit

0.059ng/mL

gABRa2

17916Q10078ROS

This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

The microtiter plate provided in this kit has been pre-coated with an antibody specific to this index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to this index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain this index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of this index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.

12 months

P47869