Human Heat Shock 70kDa Protein 1A (HSPA1A) ELISA Kit

Hersteller DlDevelop
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 96T
ArtNr DL-HSPA1A-Hu-96T
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Short description
For quantitatively determining the concentration in the customer's samples
Sensitivity
0.54ng/mL
Detection range
1.56-100ng/mL
Antigen
HSPA1A
Certified
CE&ISO
ISO- certification number
17916Q10078ROS
Cross Reaction
This assay has high sensitivity and excellent specificity for detection of this index.
No significant cross-reactivity or interference between this index and analogues was observed.
Intended Use
This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Kit Content
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 2
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120?L Assay Diluent A 1×12mL
Detection Reagent B 1×120?L Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Principle of the Elisa
The microtiter plate provided in this kit has been pre-coated with an antibody specific to this index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to this index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain this index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of this index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage and Stability
1.For unopened kit: All the reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer (30 × concentrate) and the Stop Solution should be stored at 4oC upon receipt while the others should be at -20oC.

2.For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Reproducibility
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Experimental method
Sandwich
Shelf life
12 months
Gene ID
3303
UniProtKB
P0DMV8
Menge: 96T
Lieferbar: In stock
Listenpreis: 681,94 €
Rabatt: -10,0%
Preis: 613,75 €
Sie sparen: 68,19 €
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