Vergleich

Mouse Adrenocorticotropic Hormone (ACTH) ELISA Kit

Hersteller DlDevelop
Kategorie
Typ Elisa-Kit
Specific against Mouse
Menge 96T
ArtNr DL-ACTH-Mu-96T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Short description
For quantitatively determining the concentration in the customer's samples
Sensitivity
6.39pg/mL
Detection range
15.625-1000pg/mL
Antigen
ACTH
Certified
CE&ISO
ISO- certification number
17916Q10078ROS
Cross Reaction
This assay has high sensitivity and excellent specificity for detection of this index.
No significant cross-reactivity or interference between this index and analogues was observed.
Intended Use
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Kit Content
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 2
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×70?L Assay Diluent A 1×12mL
Detection Reagent B 1×120?L Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Principle of the Elisa
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to this index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled this index and unlabeled this index (Standards or samples) with the pre-coated antibody specific to this index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of this index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of this index in the sample.
Storage and Stability
1.For unopened kit: All the reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer (30 × concentrate) and the Stop Solution should be stored at 4oC upon receipt while the others should be at -20oC.

2.For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Reproducibility
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Experimental method
Competitive inhibition
Shelf life
12 months

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Menge: 96T
Lieferbar: In stock
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