SRF (phospho Ser99) Cell Based ELISA Kit

Store at + 4°C. Stable for 6 months.

This SRF (phospho Ser99) Cell Based ELISA Kit allows for the detection of SRF (phospho Ser99) and the effects that certain stimulation conditions have on SRF (phospho Ser99) expression in different cell lines. Qualitative determination of SRF (phospho Ser99) concentration is achieved by an indirect ELISA format. In essence, SRF (phospho Ser99) is captured by Anti-SRF (phospho Ser99) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-SRF Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Fluorometric