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Human Attractin ELISA Kit PicoKine®

Human Attractin ELISA Kit PicoKine®

ArtNr	BOS-EK2135
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Metabolism Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<20 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ATRN,KIAA0548,MGCA,Attractin,DPPT-L,Mahogany homolog
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	ATRN
Gene Full Name	attractin
Manufacturer - Specificity	Natural and recombinant human ATRN
Background	Attractin is a protein that in humans is encoded by the ATRN gene. This gene encodes both membrane-bound and secreted protein isoforms. A membrane-bound isoform exhibits sequence similarity with the mouse mahogany protein, a receptor involved in controlling obesity. A secreted isoform is involved in the initial immune cell clustering during inflammatory responses that may regulate the chemotactic activity of chemokines.
Immunogen	Expression system for standard: CHO; Immunogen sequence: A84-Q1272
Protein Name	Attractin
Description	Human Attractin ELISA Kit PicoKine® (96 Tests). Quantitate Human ATRN in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine
Sample Data	Concentration (ng/ml) \| 0 \| 0.78 \| 1.56 \| 3.12 \| 6.25 \| 12.5 \| 25 \| 50 O.D. \| 0.003 \| 0.165 \| 0.321 \| 0.560 \| 0.936 \| 1.457 \| 1.926 \| 2.344
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean(pg/ml) \| 1352 \| 5864 \| 26013 \| 1363 \| 6055 \| 26542 Standard deviation \| 85.18 \| 363.57 \| 1586.79 \| 94.05 \| 387.52 \| 1353.64 CV(%) \| 6.3% \| 6.2% \| 6.1% \| 6.9 \| 6.4% \| 5.1%
Reproducibility	To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 1352 \| 1388 \| 1425 \| 1501 \| 1417 \| 72.24 \| 5.1% Sample 2 \| 5864 \| 5941 \| 5263 \| 5554 \| 5656 \| 214.91 \| 3.8% Sample 3 \| 26013 \| 27512 \| 26944 \| 25414 \| 26471 \| 1191.18 \| 4.5% *number of samples for each test n=16.
Capture antibody and Detection antibody	a monoclonal antibody from mouse\|a biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	50 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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BOS-EK2135-datasheet.pdf

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