Human CFHR4 ELISA Kit PicoKine®

PicoKine® ELISA Kits

CFHR4

Complement factor H-related protein 4 is a protein that in humans is encoded by the CFHR4 gene. This gene is a member of the complement factor H (CFH) gene family, and encodes one of the 5 CFH-related (CFHR) proteins. These 5 genes are closely linked to the CFH gene on chromosome 1q31-q32. The CFHRs are secreted plasma proteins synthesized primarily by the hepatocytes, and composed of highly-related short consensus repeats (SCRs). This protein enhances the cofactor activity of CFH, and is involved in complement regulation. It can associate with lipoproteins and may play a role in lipid metabolism. Alternatively spliced transcript variants encoding different isoforms (varying in the number of SCRs) have been described for this gene.

Human CFHR4 ELISA Kit PicoKine® (96 Tests). Quantitate Human CFHR4 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Concentration (pg/ml) | 0 | 312 | 625 | 1, 250 | 2, 500 | 5, 000 | 10, 000 | 20, 000
O.D. | 0.029 | 0.072 | 0.143 | 0.302 | 0.591 | 0.961 | 1.431 | 2.167

Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 452 | 461 | 470 | 482 | 466 | 21.45 | 4.6%
Sample 2 | 1663 | 1653 | 1743 | 1732 | 1698 | 54.33 | 3.2%
Sample 3 | 4416 | 4612 | 4741 | 4674 | 4611 | 198.26 | 4.3%
*number of samples for each test n=16.

Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4

Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.