Mouse CD40/TNFRSF5 PicoKine® Quick ELISA Kit

Quick ELISA Kits

Cd40

CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

Mouse CD40/TNFRSF5 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse CD40/TNFRSF5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1, 000
O.D. | 0.033 | 0.159 | 0.249 | 0.448 | 0.785 | 1.405 | 1.981 | 2.319

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 28 | 31 | 34 | 35 | 32 | 2.05 | 6.4%
Sample 2 | 198 | 188 | 199 | 180 | 191 | 7.27 | 3.8%
Sample 3 | 433 | 412 | 455 | 510 | 453 | 13.12 | 2.9%
*number of samples for each test n=16.

Description|Quantity|Volume
Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells
Standard||2
Antibody A|1|3ml
Antibody B|1|3ml
Sample Diluent|1|15ml
TBS-T Wash Buffer (25x)|1|12ml
Color Developing Reagent (TMB)|1|10ml
Stop Solution|1|10ml
Adhesive Plate Sealers|2|Piece

heparin or EDTA

There is no detectable cross-reactivity with other relevant proteins.