ELISA Kit for Diazepam Binding Inhibitor (DBI)

Serum, plasma and other biological fluids.

This assay has high sensitivity and excellent specificity for detection of Diazepam Binding Inhibitor (DBI)
No significant cross-reactivity or interference between Diazepam Binding Inhibitor (DBI) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Diazepam Binding Inhibitor (DBI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Diazepam Binding Inhibitor (DBI) and unlabeled Diazepam Binding Inhibitor (DBI) (Standards or samples) with the pre-coated antibody specific for Diazepam Binding Inhibitor (DBI). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Diazepam Binding Inhibitor (DBI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Diazepam Binding Inhibitor (DBI) in the sample.