Anti-Omalizumab Neutralizing Antibody ELISA kit

Used for the quantitative determination of Anti-Omalizumab Neutralizing Antibody concentration in serum and plasma.

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PRINCIPLE OF THE ASSAYThis assay employs the quantitative competitive enzyme immunoassay technique. Omalizumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human IgE and then pipetted into the wells. Anti-Omalizumab Neutralizing Antibody in the sample competitively binds to Omalizumab with the biotin-labeled Human IgE. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Omalizumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.