Multiplex Assay Kit for Intercellular Adhesion Molecule 4 (ICAM4) ,etc. by FLIA (Flow Luminescence Immunoassay)

FLIA Kit

3h

This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 4 (ICAM4) , etc. by FLIA (Flow Luminescence Immunoassay).

No significant cross-reactivity or interference between Intercellular Adhesion Molecule 4 (ICAM4) , etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is
proportional to the concentration of analytes of interest in the sample.