Unfractionated Heparin (UFH) ELISA for Plasma

Heparin is a glycosaminoglycan with alternating uronic acid and aminoglycoside units. It is an anticoagulant used either in its native unfractionated form (UFH) MW ~, 16 kD or in various partially depolymerized forms (LMWH) of 4-8 kD. The heparin-ELISA product number K-2100 is a quantitative enzyme-linked assay designed for the in vitro measurement of unfractionated heparin levels in plasma. This assay measures heparin directly using a heparin binding protein which has been conjugated to HRP. The heparin-ELISA is a competitive assay in which the colorimetric signal is inversely proportional to the amount of heparin present in the sample. Samples to be assayed are first mixed with the Detector-Enzyme Conjugate in wells of the heparin coated plate. Heparin in the sample competes with heparin bound to the plate for binding of the Detector-Enzyme Conjugate. The concentration of heparin in the sample is determined using a standard curve of known amounts of heparin. For best results, the heparin used for the standard curve should match the type of heparin being assayed.

Pipettes (8-channel multipipettor recommended) Absorbance microplate reader Plate Cover Fresh Normal Human Plasma