Human IL-35 ELISA Kit

Blue ice

0.21 100 pg/ml

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection ofhuman IL-35. The Human IL-35 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin 35 (IL-35) is an IL-12 family cytokine produced by regulatory, but not effector, T-cells and plays a role in immune suppression. It is a dimeric protein composed of IL-12alpha and IL-27beta chains, which are encoded by two separate genes called IL12A and EBI3, respectively. Secreted by regulatory T-cells (Tregs), IL-35 suppresses inflammatory responses of immune cells. IL-35 is not constitutively expressed in tissues, but the gene encoding IL-35 is transcribed by vascular endothelial cells, smooth muscle cells and monocytes after activation with proinflammatory stimuli. Studies in mice show the absence of either IL-35 chain from regulatory Tregs reduces the cells' ability to suppress inflammation; this has been observed during cell culture experiments and using an experimental model for inflammatory bowel disease. To produce its suppressive effects, IL-35 has selective activities on different T-cell subsets; it induces proliferation of Treg cell populations but reduces activity of Th17 cellpopulations.

Sandwich

ELISA detection, IL-35, Sandwich ELISA assay

4CC

100uL

Human IL-35 ELISA kit

0.02% sodium azide

pipettes, tubes, reader

NM_145659/NM_005755

Online product mannual

Online product mannual

Online product mannual

Online product mannual