Human AngIII(Angiotensin III) ELISA Kit

Competitive Inhibition

Endocrinology; Cardiovascular biology; Hormone metabolism

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Angiotensin III (AngIII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Angiotensin III (AngIII) and unlabeled Angiotensin III (AngIII) (Standards or samples) with the pre-coated antibody specific to Angiotensin III (AngIII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin III (AngIII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Angiotensin III (AngIII) in the sample.