Blood/Cultured Cell Total RNA Maxi Kit (10prep)

1. Make sure everything is RNase-free when handling RNA.
2. Buffers provided in this system contain irritants. Wear gloves and lab
coat when handling hese buffers.
3. Pipet a required volume of FARB Buffer to another RNase-free container
and add 10 ul ß-mercaptoethanol (ß-ME) per 1ml FARB Buffer before use.
4. Add required volume of RNase-free ethanol (96~100%) to Wash Buffer 2
as bottle indicated when first open.
5. Dilute RNase-free DNase 1 in reaction buffer (150mM NaCl, 1 mM MgCl2,
10 mM Tris HCl, pH 7.5) to final conc. = 2KU/ml. (1 ml /preparation)
6. Use a centrifuge with a swinging bucket rotor for 15ml (Midi) or 50ml (Maxi)
in all centrifugation steps. The maximum speed should be 3500-5000 rpm
or 3000-5000 x g.