Plant Total RNA Mini Kit (100prep), for Woody Plant

Store at room temperature (15~ 25 C) for 2 year.

1. Make sure everything is RNase-free when handling RNA.
2. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
3. Pipet a required volume of FARB-1 Buffer to another RNase-free container and add 10 ul of
ß-mercaptoethanol (ß-ME) per 1ml FARB-1 Buffer before use.
4. Add required volume of ethanol(96-100%) as bottle indicated to Wash Buffer 2 when first open.
5. Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10mM MnCl2, 20mM Tris-HCl, pH7.0 at 25 C)
to final conc.= 0.5U/ul.

50 ul

up to 100 mg plant tissue or 1 X 10 plant cells