Soil DNA Isolation Mini Kit (50prep)

1. Buffers provided in this system contain irritants. Wear gloves and
lab coat when handling these buffers.
2. Check SDE1 Buffer before use, Warm SDE1 Buffer at 60C for 10
minutes if any precipitate formd.
3. Add indicated volume of ethanol (96-100%) to Wash Buffer before use.
4. Prepare a heating block or a water bath to 70 C. If DNA is isolated from
gram positive bacteria, prepare a heating block or a water bath to 95 C
for another incubation.
5. All centrifuge steps are done at full speed (~18, 000 x g) in a microcentrifuge.
6. Preheat Elution Buffer or ddH2O to 60C for elution step.

spin column (silica membrane)

0.25 ~ 0.5 g