Protein Crystallization (PCS-1)

Completely revised and updated, Protein Crystallization, 2nd Edition is a greatly expanded follow-up to the best-selling 1st edition. Completely new chapters on high-throughput methods, mass spectrometry, microcalorimetry, counterdiffusion, heavy-atom derivatization, selenomethionine-labeling, rational strategies for crystallization, and protein modification to improve crystallization. Updated chapters on formulation of the protein before crystallization, characterization of the protein by dynamic light scattering, classic methods and the phase diagram, seeding, and cryoprotection of the crystals. Laboratory exercises are included which can be used for teaching purposes in protein crystallization or crystallography courses. The exercises have been tested in the Practical Protein Crystallization courses that have been running since 1994 at Uppsala University (Sweden), as well as courses elsewhere. The book is divided into four separate sections: Methods, Tools and Strategies, Laboratory Exercises, and an A-Z Glossary. There are 30 color plates for identifying phenomena found in crystallization drops. As in the first edition, the emphasis of the book is on practical problems and questions encountered at the lab bench.