BRAF Antibody, mAb, Mouse

USA

-20°C

BRAF (V-raf murine sarcoma viral oncogene homolog B1) is the main effector recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites, Ser364, Ser428, and Thr439. B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK). It has a long amino-terminal region, which is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf can propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testes. Defects in BRAF are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma, melanocytic nevi, primary cutaneous melanomas, and colorectal cancers.