Tau Antibody (Phospho-Thr217), pAb, Rabbit

Rabbit IgG

The antibody is stable for 2-3 weeks if stored at 2-8C. For long term storage, aliquot and store at -20C or below. Avoid repeated freezing and thawing cycles.

GenScript Rabbit Anti-Tau (Phospho-Thr217) Polyclonal Antibody detects endogenous levels of Tau only when phosphorylated at threonine 217.

Tau is a microtubule-associated phosphoprotein (MAP), localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, which range from 352 to 441 amino acids. The tau variants differ from each other by the presence of either three or four repeat-regions in the carboxy-terminal part of the molecule and the absence or presence of one or two inserts in the amino-terminal part. Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3beta transfection phosphorylates serines 199, 202, 235, 396, 404 and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of Tau. Phosphorylation on these sites reduces the ability of a given Tau species to promote microtubule self-assembly. Hyperphosphorylated Tau is the major protein of the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer’s disease (AD). These PHFs are also found in the lesions of other central nervous system disorders. GenScript, Rabbit Anti-Tau (Phospho-Thr217) Polyclonal Antibody, is developed in rabbit using a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr217 of human Tau.

The antibody is purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide and cross-adsorbed with the corresponding non-phosphopeptide.