Tau Antibody (Phospho-Thr205), pAb, Rabbit

USA

GenScript Rabbit Anti-Tau (Phospho-Thr205) Polyclonal Antibody detects endogenous levels of tau protein only when phosphorylated at threonine 205.

Tau is a microtubule-associated phosphoprotein (MAP), localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, which range from 352 to 441 amino acids in size. These tau variants differ from each other in the presence of either three or four repeat-regions in the carboxy-terminal part of the molecule and the presence or absence of one or two inserts in the amino-terminal portion. Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3beta transfection phosphorylates serines 199, 202, 235, 396, 404 and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of tau. Phosphorylation on these sites reduces the ability of a given tau species to promote microtubule self-assembly. Hyperphosphorylated tau is the major protein of the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer’s disease (AD). These PHFs are also found in the lesions of other central nervous system disorders. GenScript, Rabbit Anti-Tau (Phospho-Thr205) Polyclonal Antibody, is developed in rabbit hosts using a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding threonine 205 of human tau.

Affinity chromatography using phosphopeptide, cross-adsorbed with the corresponding non-phosphopeptide

55 kD