ERK1/2 Antibody (Phospho-Thr202/Tyr204), pAb, Rabbit

USA

GenScript Rabbit Anti-ERK1/2 (Phospho-Thr202/Tyr204) Polyclonal Antibody detects endogenous levels of human and rat ERK1 and ERK2 when dually phosphorylated at Thr202 and Tyr204 of ERK1 (Thr185 and Tyr187 of ERK2), and singly phosphorylated at Tyr204. The antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase. Predicted to react with mouse ERK1 and ERK2 protein according to sequence homology.

ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) includes two closely related isoforms ERK1 and ERK2 (p44 and p42 MAP Kinase). ERK1/2 signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. The downstream effects of ERK1/2 signaling pathway are linked to the regulation of cell growth and differentiation as well as the cytoskeleton. Downstream targets of ERK1/2 contain p90RSK and the transcription factor Elk-1. ERK1 and ERK2 are phosphorylated within the activation loop on both a threonine and a tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1/2. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases. GenScript, Rabbit Anti-ERK1/2 (Phospho-Thr202/Tyr204) Polyclonal Antibody, is developed in rabbit using a KLH-coupled synthetic dual phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human ERK1 (Swiss Prot: P27361) and ERK2 (Swiss Prot: P28482).

Affinity chromatography using dual phosphopeptide, cross -adsorbed with corresponding single phosphopetide and non-phosphopeptide

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.