FEN-1 Antibody, pAb, Rabbit

USA

GenScript Rabbit Anti-FEN-1 Polyclonal Antibody detects endogenous levels of human, mouse, and rat FEN-1 protein. Positive Control: Hela, mouse brain and rat brain.

Flap Endonuclease 1 (FEN-1) is a protein that plays a role in lagging strand DNA replication, base excision repair, homologous recombination, and re-initiation of stalled replication forks. FEN-1 is localized at the telomere and contributes to telomere stability by ensuring efficient telomere replication. FEN-1 contains a small surface-exposed hydrophobic wedge and a 1-ntbinding pocket, which provides specificity for double-flap structures and appears to contribute to the positioning of the ssDNA 50 flap near the active site. Acetylation of the FEN-1 C-terminus by p300 significantly reduces DNA binding and nuclease activity of FEN-1. PCNA is able to stimulate both acetylated and unacetylated FEN-1 activity. GenScript, Rabbit Anti-FEN-1 Polyclonal Antibody, is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human FEN-1 protein (Swiss Prot: P39748).

Immunoaffinity chromatography