HEK293/Gα15 Stable Cell Line

Cellular Analysis

-80°C

GPCR Cell Lines

1.HEK293/Galpha15 HEK293/Galpha15 is a HEK293 cell line stably expressing the Galpha15 alpha subunit protein which a Gq protein. It is used as a host cell for transfection expression of Gs and Gi/o -coupled receptors, the constitutively expressed Galpha15 protein in the cells allows many transfected receptors which normally stimulate/inhibit the cAMP pathway, to couple to Gq signal transduction and mobilize intracellular calcium. The cell line carries the puromycin resistance gene and is resistant to puromycin

2.The sequence of Galpha15 ATGGCCCGCTCGCTGACCTGGCGCTGCTGCCCCTGGTGCCTGACGGAGGATGAG AAGGCCGCCGCCCGGGTGGACCAGGAGATCAACAGGATCCTCTTGGAGCAGAAGA AGCAGGACCGCGGGGAGCTGAAGCTGCTGCTTTTGGGCCCAGGCGAGAGCGGGAA GAGCACCTTCATCAAGCAGATGCGGATCATCCACGGCGCCGGCTACTCGGAGGAG GAGCGCAAGGGCTTCCGGCCCCTGGTCTACCAGAACATCTTCGTGTCCATGCGGGC CATGATCGAGGCCATGGAGCGGCTGCAGATTCCATTCAGCAGGCCCGAGAGCAAGC ACCACGCTAGCCTGGTCATGAGCCAGGACCCCTATAAAGTGACCACGTTTGAGAAG CGCTACGCTGCGGCCATGCAGTGGCTGTGGAGGGATGCCGGCATCCGGGCCTACTA TGAGCGTCGGCGGGAATTCCACCTGCTCGATTCAGCCGTGTACTACCTGTCCCACCT GGAGCGCATCACCGAGGAGGGCTACGTCCCCACAGCTCAGGACGTGCTCCGCAGC CGCATGCCCACCACTGGCATCAACGAGTACTGCTTCTCCGTGCAGAAAACCAACCT GCGGATCGTGGACGTCGGGGGCCAGAAGTCAGAGCGTAAGAAATGGATCCATTGTT TCGAGAACGTGATCGCCCTCATCTACCTGGCCTCACTGAGTGAATACGACCAGTGC CTGGAGGAGAACAACCAGGA GAACCGCATGAAGGAGAGCCTCGCATTGTTTGGGACTATCCTGGAACTACCCT GGTTCAAAAGCACATCCGTCATCCTCTTTCTCAACAAAACCGACATCCTGGAG GAGAAAATCCCCACCTCCCACCTGGCTACCTATTTCCCCAGTTTCCAGGGCC CTAAGCAGGATGCTGAGGCAGCCAAGAGGTTCATCCTGGACATGTACACGAG GATGTACACCGGGTGCGTGGACGGCCCCGAGGGCAGCAAGAAGGGCGCACG ATCCCGACGCCTCTTCAGCCACTACACATGTGCCACAGACACACAGAACATC CGCAAGGTCTTCAAGGACGTGCGGGACTCGGTGCTCGCCCGCTACCTGGACG AGATCAACCTGCTGTGA