Hersteller |
GenScript |
Kategorie |
|
Typ |
Proteins |
Specific against |
Human |
Menge |
20ug |
ArtNr |
Z02749-20 |
Eclass 6.1 |
34160400 |
Eclass 9.0 |
42020190 |
Lieferbar |
|
Product Line |
Cytokine, Chemokines & Growth Factors |
Description |
Betacellulin (BTC) is a member of the EGF family of cytokines that also includes EGF, TGF-a, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. At the amino acid sequence level, human mature BTC protein exhibits 80% identity with mouse BTC protein. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. |
M.W. |
Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein. |
Purity |
>98% by SDS-PAGE and HPLC analyses. |
Endotoxin Level |
Less than 0.2EU/ug of rHuBetacellulin as determined by LAL method. |
Specific Activity |
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is ≤, 0.05 ng/ml, corresponding to a specific activity of ≥, 2.0 x 107units/mg. |
Storage |
This lyophilized preparation is stable at 2-8 C, but should be kept at -20 C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 C to -70 C. Avoid repeated freeze/thaw cycles. |
Formulation |
Lyophilized from a 0.2µ, m filtered concentrated, solution in PBS, pH 7.4. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °, C. Further dilutions should be made in appropriate buffered solutions. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Usage |
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
Sequence |
DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY |