MOUSE ANTI HUMAN CD154 (SOLUBLE)

CD154 (SOLUBLE)

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Recognises human CD154 a 32kD glycoprotein also known as CD40 ligand. It is a member of the TNF family of molecules. It binds to CD40 and acts as a co-stimulatory molecule by activating antigen presenting cells such as B cells. While CD154 is mostly found expressed on activated T cells

Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Ref. 10Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa actin polymerization and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. Ref. 10Subunit structureHomotrimer. Subcellular locationCell membrane; Single-pass type II membrane protein. CD40 ligand soluble form: Secreted. Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes. Post-translational modificationThe soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated. Ref. 9Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin the overall prognosis is rather poor with a death rate of about 10% before adolescence. Ref. 3Ref. 13Ref. 14Ref. 15Ref. 16Ref. 17Ref. 18Ref. 19Ref. 20Ref. 21Sequence similaritiesBelongs to the tumor necrosis factor family. [1] \" Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins. \" Tashiro K. Tada H. Heilker R. Shirozu M. Nakano T. Honjo T. Science 261:600-603(1993) [PubMed: 8342023] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA). [2] \" Molecular cloning of TPAR1 a gene whose expression is repressed by the tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA). \" Jiang W. Zhou P. Kahn S. M. Tomita N. Johnson M. D. Weinstein I. B. Exp. Cell Res. 215:284-293(1994) [PubMed: 7982471] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA). [3] \" Molecular cloning and structure of a pre-B-cell growth-stimulating factor. \" Nagasawa T. Kikutani H. Kishimoto T. Proc. Natl. Acad. Sci. U. S. A. 91:2305-2309(1994) [PubMed: 8134392] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA). [4] Nomura M. Nakata Y. Uzawa A. Nose M. Akashi M. Suzuki G. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA). Strain: AKR/J. [5] \" The transcriptional landscape of the mammalian genome. \" Carninci P. Kasukawa T. Katayama S. Gough J. Frith M. C. Maeda N. Oyama R. Ravasi T. Lenhard B. Wells C. Kodzius R. Shimokawa K. Bajic V. B. Brenner S. E. Batalov S. Forrest A. R. Zavolan M. Davis M. J. Wilming L. G. Aidinis V. Allen J. E. Ambesi-Impiombato A. Apweiler R. Aturaliya R. N. Bailey T. L. Bansal M. Baxter L. Beisel K. W. Bersano T. Bono H. Chalk A. M. Chiu K. P. Choudhary V. Christoffels A. Clutterbuck D. R. Crowe M. L. Dalla E. Dalrymple B. P. de Bono B. Della Gatta G. di Bernardo D. Down T. Engstrom P. Fagiolini M. Faulkner G. Fletcher C. F. Fukushima T. Furuno M. Futaki S. Gariboldi M. Georgii-Hemming P. Gingeras T. R. Gojobori T. Green R. E. Gustincich S. Harbers M. Hayashi Y. Hensch T. K. Hirokawa N. Hill D. Huminiecki L. Iacono M. Ikeo K. Iwama A. Ishikawa T. Jakt M. Kanapin A. Katoh M. Kawasawa Y. Kelso J. Kitamura H. Kitano H. Kollias G. Krishnan S. P. Kruger A. Kummerfeld S. K. Kurochkin I. V. Lareau L. F. Lazarevic D. Lipovich L. Liu J. Liuni S. McWilliam S. Madan Babu M. Madera M. Marchionni L. Matsuda H. Matsuzawa S. Miki H. Mignone F. Miyake S. Morris K. Mottagui-Tabar S. Mulder N. Nakano N. Nakauchi H. Ng P. Nilsson R. Nishiguchi S. Nishikawa S. Nori F. Ohara O. Okazaki Y. Orlando V. Pang K. C. Pavan W. J. Pavesi G. Pesole G. Petrovsky N. Piazza S. Reed J. Reid J. F. Ring B. Z. Ringwald M. Rost B. Ruan Y. Salzberg S. L. Sandelin A. Schneider C. Schoenbach C. Sekiguchi K. Semple C. A. Seno S. Sessa L. Sheng Y. Shibata Y. Shimada H. Shimada K. Silva D. Sinclair B. Sperling S. Stupka E. Sugiura K. Sultana R. Takenaka Y. Taki K. Tammoja K. Tan S. L. Tang S. Taylor M. S. Tegner J. Teichmann S. A. Ueda H. R. van Nimwegen E. Verardo R. Wei C. L. Yagi K. Yamanishi H. Zabarovsky E. Zhu S. Zimmer A. Hide W. Bult C. Grimmond S. M. Teasdale R. D. Liu E. T. Brusic V. Quackenbush J. Wahlestedt C. Mattick J. S. Hume D. A. Kai C. Sasaki D. Tomaru Y. Fukuda S. Kanamori-Katayama M. Suzuki M. Aoki J. Arakawa T. Iida J. Imamura K. Itoh M. Kato T. Kawaji H. Kawagashira N. Kawashima T. Kojima M. Kondo S. Konno H. Nakano K. Ninomiya N. Nishio T. Okada M. Plessy C. Shibata K. Shiraki T. Suzuki S. Tagami M. Waki K. Watahiki A. Okamura-Oho Y. Suzuki H. Kawai J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA). Strain: C57BL/6J. Tissue: Embryo and Kidney. [6] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA). Strain: C57BL/6J. Tissue: Mammary gland.