MOUSE ANTI HUMAN FACTOR VIII

FACTOR VIII

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Recognises human Factor VIII an essential blood coagulation factor. Whilst circulating in the blood it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites dissociates from the complex and interacts with Factor IXa in the presence of calcium ions and phospholipids to convert Factor X to the activated Factor Xa which activates thrombin. Thrombin cleaves fibrinogen into fibrin which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A a disorder characterised by the body\' s inability to control blood clotting. This could result in severe blood loss even with minor injuries. Clone RFF-VIIIC/5 recognises an epitope towards the C-terminus of full-length Factor VIII. It also recognises the 80kDa and 70kDa cleavage products. Recommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L) 1. Thome M. et al (1997) Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 386:517-521. 2. Irmler M. et al (1997) Inhibition of death receptor signals by cellular FLIP. Nature 388:190-195. 3. Shu H. B. et al (1997) Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 6:751-763. 4. Hu S. et al (1997) I-FLICE a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J. Biol. Chem. 272:17255-17257. [1] \" Casper is a FADD- and caspase-related inducer of apoptosis. \" Shu H. -B. Halpin D. R. Goeddel D. V. Immunity 6:751-763(1997) [PubMed: 9208847] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 13 AND 14) MUTAGENESIS OF TYR-360. Tissue: Embryonic kidney and Umbilical vein endothelial cell. [2] \" MRIT a novel death-effector domain-containing protein interacts with caspases and BclXL and initiates cell death. \" Han D. K. M. Chaudhary P. M. Wright M. E. Friedman C. Trask B. J. Riedel R. T. Baskin D. G. Schwartz S. M. Hood L. Proc. Natl. Acad. Sci. U. S. A. 94:11333-11338(1997) [PubMed: 9326610] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3). [3] \" Inhibition of death receptor signals by cellular FLIP. \" Irmler M. Thome M. Hahne M. Schneider P. Hofmann K. Steiner V. Bodmer J. -L. Schroeter M. Burns K. Mattmann C. Rimoldi D. French L. E. Tschopp J. Nature 388:190-195(1997) [PubMed: 9217161] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Peripheral blood lymphocyte. [4] \" FLAME-1 a novel FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-induced apoptosis. \" Srinivasula S. M. Ahmad M. Ottilie S. Bullrich F. Banks S. Wang Y. Fernandes-Alnemri T. Croce C. M. Litwack G. Tomaselli K. J. Armstrong R. C. Alnemri E. S. J. Biol. Chem. 272:18542-18545(1997) [PubMed: 9228018] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 8; 9 AND 10) MUTAGENESIS OF ASP-376. Tissue: T-cell. [5] \" I-FLICE a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. \" Hu S. Vincenz C. Ni J. Gentz R. Dixit V. M. J. Biol. Chem. 272:17255-17257(1997) [PubMed: 9211860] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Umbilical vein endothelial cell. [6] Hu S. Dixit V. M. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 4; 5; 6 AND 7). [7] \" CASH a novel caspase homologue with death effector domains. \" Goltsev Y. V. Kovalenko A. V. Arnold E. Varfolomeev E. E. Brodianskii V. M. Wallach D. J. Biol. Chem. 272:19641-19644(1997) [PubMed: 9289491] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Skin fibroblast. [8] \" CLARP a death effector domain-containing protein interacts with caspase-8 and regulates apoptosis. \" Inohara N. Koseki T. Hu Y. Chen S. Nunez G. Proc. Natl. Acad. Sci. U. S. A. 94:10717-10722(1997) [PubMed: 9380701] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Colon carcinoma. [9] \" Cell death attenuation by \' Usurpin\' a mammalian DED-caspase homologue that precludes caspase-8 recruitment and activation by the CD-95 (Fas APO-1) receptor complex. \" Rasper D. M. Vaillancourt J. P. Hadano S. Houtzager V. M. Seiden I. Keen S. L. C. Tawa P. Xanthoudakis S. Nasir J. Martindale D. Koop B. F. Peterson E. P. Thornberry N. A. Huang J. MacPherson D. P. Black S. C. Hornung F. Lenardo M. J. Hayden M. R. Roy S. Nicholson D. W. Cell Death Differ. 5:271-288(1998) [PubMed: 10200473] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 11 AND 12). Tissue: Kidney. [10] \" Cloning and characterization of three novel genes ALS2CR1 ALS2CR2 and ALS2CR3 in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2. \" Hadano S. Yanagisawa Y. Skaug J. Fichter K. Nasir J. Martindale D. Koop B. F. Scherer S. W. Nicholson D. W. Rouleau G. A. Ikeda J. -E. Hayden M. R. Genomics 71:200-213(2001) [PubMed: 11161814] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].