MOUSE ANTI HUMAN CD98

CD98

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatent.

HOON pre-B leukaemia cell line

Required for the function of light chain amino-acid transporters. Involved in sodium-independent high-affinity transport of large neutral amino acids such as phenylalanine tyrosine leucine arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger following an antiport mechanism for amino acid transport influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1 is also involved in the transport of L-DOPA across the blood-brain barrier and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D L-homocysteine complexes and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8 involved in the cellular activity of small molecular weight nitrosothiols via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1 regulates the transport activity LAT2 in polarized intestinal cells by generating and delivering intracellular signals. When associated with SLC7A5 plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. Ref. 6Ref. 12Ref. 13Ref. 14Ref. 15Ref. 16Ref. 17Ref. 19Ref. 20Ref. 22Ref. 23Ref. 25Ref. 26Ref. 28Ref. 29Subunit structureDisulfide-linked heterodimer of a glycosylated heavy chain and a non-glycosylated light chain (SLC7A5 SLC7A6 SLCA7A7 SLC7A8 SLC7A10 or SLCA7A11). Colocalizes with cadherins By similarity. Interacts with FAM57A/CT120 and ICAM1. Constitutively and specifically associates with beta-1 integrins (alpha-2/beta-1 alpha-3/beta-1 alpha-5/beta-1 and alpha-6/beta-1) but minimally with alpha-4/beta-1. Ref. 6Ref. 12Ref. 13Ref. 14Ref. 16Ref. 17Ref. 19Ref. 23Ref. 25Ref. 26Ref. 29Ref. 18Ref. 21Ref. 40Subcellular locationApical cell membrane; Single-pass type II membrane protein. Melanosome.

Arginine uptake is inhibited by increasing concentrations of leucine in the presence of Na+. Sequence similaritiesBelongs to the SLC3A transporter family. Biophysicochemical properties Kinetic parameters:KM=295 & micro; M for glutamine (in the presence of NaCl) Ref. 15KM=236 & micro; M for leucine (in the presence of NaCl)KM=120 & micro; M for arginine (in the presence of NaCl)KM=138 & micro; M for arginine (in the absence of NaCl)Mass spectrometryMolecular mass is 57944. 93 Da from positions 1 - 529. Determined by MALDI. Ref. 24