MOUSE ANTI HUMAN CD13

GWB-2F2B86

IgG1Species Cross Reactivity: Reacts with: Rhesus MonkeyN. B. Antibody reactivity and working conditions may vary between species.

Phosphate buffered saline pH7. 4

Human AML cells

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells macrophages granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Ref. 11Ref. 13Ref. 14Ref. 15Ref. 17Ref. 18Ref. 19Ref. 20Ref. 22Catalytic activityRelease of an N-terminal amino acid Xaa-|-Yaa- from a peptide amide or arylamide. Xaa is preferably Ala but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue the two may be released as an intact Xaa-Pro dipeptide. Cofactor: Binds 1 zinc ion per subunit By similarity. Subunit structureHomodimer. Interacts with the S1 domain of HCoV-229E spike protein. Ref. 11Ref. 9Ref. 23Subcellular locationCell membrane; Single-pass type II membrane protein. Cytoplasm & rsaquo; cytosol Potential.

Estradiol and IL-8 decrease enzymatic activity in vitro in endometrial stromal cells by 40% and 30% respectively. Ref. 20

Found to serve as a receptor for tumor-homing peptides more specifically NGR peptides. It could serve thus as a target for delivering drugs into tumors. Concentration in human hepatic bile varies from 17. 3 to 57. 6 micrograms/ml. Sequence similaritiesBelongs to the peptidase M1 family. 1. Southern J. A. et al. (1991) Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72: 1551-1557. 2. Hanke T. et al. (1992) Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J. Gen. Virol. 73: 653-660. 3. Randall R. E. et al. (1993) Two-tag purification of recombinant proteins for the construction of solid matrix-antibody antigen (SMAA) complexes as vaccines. Vaccine 11:1247-1252. 4. Randall R. E. et al. (1994) Purification of antibody-antigen complexes containing recombinant SIV proteins; comparison of antigen and antibody-antigen complexes for immune priming. Vaccine 12: 351-358. 5. Hanke T. et al. (1995) Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding J. Virol. Methods 149-156. 6. Jaffray E. et al. (1995) Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65 Mol. Cell. Biol. 15: 2166-2172. 7. Rodriguez M. S. et al. (1995) Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein. Mol. Cell. Biol. 15: 2413-2419. 8. Arenzana-Seisdedos F. et al. (1995) Inducible nuclear expression of newly synthesised IkBa negatively regulates DNA binding and transcriptional activities of NF-kB. Mol. Cell. Biol. 15: 2689-2696. 9. Hirst K. et al. (1994) The transcription factor the Cdk its cyclin and their regulator: directing the transcriptional response to a nutritional signal EMBO J. 13: 5410-5420. 10. Dunn C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. J. Immunol. Methods. 224: 141-150. 11. Young D. F. et al. (2001) Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells J. Virol. 75 (7): 3363 - 3370. 12. Stuart A. MacNeill (2004) The C-terminal zinc finger of the catalytic subunit of DNA polymerase {delta} is responsible for direct interaction with the B-subunit Nucleic Acids Research 32: 3005-3016 [1] \" Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5. \" Thomas S. M. Lamb R. A. Paterson R. G. Cell 54:891-902(1988) [PubMed: 3044614] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] RNA EDITING. [2] \" NP:P and NP:V interactions of the paramyxovirus simian virus 5 examined using a novel protein:protein capture assay. \" Randall R. E. Bermingham A. Virology 224:121-129(1996) [PubMed: 8862406] [Abstract]Cited for: INTERACTION WITH PROTEIN NP. [3] \" The RNA binding region of the paramyxovirus SV5 V and P proteins. \" Lin G. Y. Paterson R. G. Lamb R. A. Virology 238:460-469(1997) [PubMed: 9400618] [Abstract]Cited for: RNA-BINDING. [4] \" The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase mda-5 and inhibit its activation of the IFN-beta promoter. \" Andrejeva J. Childs K. S. Young D. F. Carlos T. S. Stock N. Goodbourn S. Randall R. E. Proc. Natl. Acad. Sci. U. S. A. 101:17264-17269(2004) [PubMed: 15563593] [Abstract]Cited for: INTERACTION WITH HUMAN IFIH1/MDA5 INTERFERON EVASION. [5] \" In vitro and in vivo specificity of ubiquitination and degradation of STAT1 and STAT2 by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2. \" Precious B. Young D. F. Andrejeva L. Goodbourn S. Randall R. E. J. Gen. Virol. 86:151-158(2005) [PubMed: 15604442] [Abstract]Cited for: UBIQUITINATION OF HUMAN STAT1 AND HUMAN STAT2. [6] \" The role of simian virus 5 V protein on viral RNA synthesis. \" Lin Y. Horvath F. Aligo J. A. Wilson R. He B. Virology 338:270-280(2005) [PubMed: 15950997] [Abstract]Cited for: FUNCTION. [7] \" Simian virus 5 V protein acts as an adaptor linking DDB1 to STAT2 to facilitate the ubiquitination of STAT1. \" Precious B. Childs K. Fitzpatrick-Swallow V. Goodbourn S. Randall R. E. J. Virol. 79:13434-13441(2005) [PubMed: 16227264] [Abstract]Cited for: INTERACTION WITH HUMAN DDB1 AND HUMAN STAT2. [8] \" Structure of DDB1 in complex with a paramyxovirus V protein: viral hijack of a propeller cluster in ubiquitin ligase. \" Li T. Chen X. Garbutt K. C. Zhou P. Zheng N. Cell 124:105-117(2006) [PubMed: 16413485] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2. 85 ANGSTROMS) OF 1-222.