Mouse Anti-Human Bcl-xL

GWB-383EB6

7B2. 5

To insure lot-to-lot consistency each batch of product is tested by immunoassay to conform to the characteristics of a standard reference reagent.

Product: Mouse Anti-Human Bcl-xLAmount Used: 2 ug/10^6 cellsMurine FL5 cells (FL5-NEO) and FL5 cells transfected with Bcl-xL expression plasmid (FL5-BCL-XL) were fixed with buffered paraformaldehyde and then permeabilized with saponin. The cells were incubated with mouse anti-human Bcl-xL followed by phycoerythrin-conjugated goat antimouse IgG and then were analyzed by flow cytometry.

0. 5 ug/ml

Potent inhibitor of cell death. Isoform Bcl-X(L) anti-apoptotic activity is inhibited by association with SIVA isoform 1. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator cytochrome c from the mitochondrial membrane. The Bcl-X(S) isoform promotes apoptosis.

Mitochondrion membrane; Single-pass membrane protein (By similarity). Nucleus envelope; Single-pass membrane protein; Cytoplasmic side (By similarity). Note=Mitochondrial membranes and perinuclear envelope (By similarity).

The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.

Belongs to the Bcl-2 family. Cohen J. J. 1991. Adv. Immunol. 50:55. Cohen J. J. and R. C. Duke. 1992. Annu. Rev. Immunol. 10:2676. Nunez G. and M. F. Clarke. 1994. Trends Cell Biol. 4:399. Cory S. 1995. Annu. Rev. Immunol. 13:513. Oltvai. Z. N. C. L. Millman and S. J. Korsmeyer. 1993. Cell 74:609. Farrow S. N. et al. 1995. Nature 374:731. Chittenden T. et al. 1995. Nature 374:733. Kiefer M. C. et al. 1995. Nature 374:736. Boise L. H. M. Gonzalez-Garcia C. E. Postema L. Ding T. Lindstein L. A. Turka X. Mao G. Nunez and C. B. Thompson 1993. Cell 74:597. Gottschalk A. R. L. H. Boise C. B. Thompson and J. Quintans. 1994. Proc. Natl. Acad. Sci. USA 91:7350. Gonzalez-Garcia M. I. Garcia L. Ding S. O\' Shea L. H. Boise C. B. Thompson and G. Nunez. 1995. Proc. Natl. Acad. Sci. USA 92:4304Dole M. G. R. Jasty M. J. Cooper C. B. Thompson G. Nunez and V. P. Castle. 1995. Can. Res. 55:2576. Shimizu S. et al. 1995. Nature 374:811. Yin X. M. Z. N. Oltvai and S. J. Korsmeyer. 1994. Nature 369:321. Cheng E. H. -Y. et al. 1996. Nature 379:554. Gottschalk A. R. L. H. Boise Z. N. Oltvai M. A. Accavitti S. J. Korsmeyer J. Quintans and C. B. Thompson. 1996. Cell Death and Differentiation 3:113. Boise L. H. 1997. Personal communication. L. Haughan. 1997. Personal communication.