PIAS2 (protein inhibitor of activated STAT. 2)

GWB-54A76F

1:2500

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase stabilizing the interaction between UBE2I and the substrate and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways including the STAT pathway the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1 thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta but not isoform PIAS2-alpha promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.

Binds SUMO1 and UBE2I. Interacts with JUN MDM2 PARK7 TP53 and TP73 isoform alpha but not TP73 isoform beta. Interacts with STAT4 following IL12 and IFN-alpha stimulation of T-cells. Interacts also with GTF2I GTF2IRD1 DAB2 and MSX2 as well as with several steroid receptors including ESR1 ESR2 NR3C1 PGR AR and with NCOA2 (By similarity). Sumoylation of a target protein seems to enhance the interaction. Binds to sumoylated ELK1. Binds DNA such as CDKN1A promoter in a sequence-specific manner. Interacts with PLAG1.

Mainly testis. Isoform 3 is expressed predominantly in adult testis weakly in pancreas embryonic testis and sperm and at very low levels in other organs. Developmental Stage: Expression of isoform 3 in adult testis is 14. 2-fold stronger than in embryonic testis.

The LXXLL motif is a transcriptional coregulator signature.

Belongs to the PIAS family.

Contains 1 SP-RING-type zinc finger. Summary: This protein is a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described. Sakurai. T. . et al. . (2004) J. Biol. Chem. 279 (15). 15505-15514.