EDF1 (endothelial differentiation-related factor 1)

Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human EDF1.

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery. thereby modulating the tran

Interacts with TBP and the transcription factor IID (TFIID) complex NR5A2 NR1H3 and PPARG. Interaction with TBP is regulated by phosphorylation. Binds NR5A1 ATF1 FOS and JUN via their conserved basic region. Binding to calmodulin is regulated by calcium and phosphorylation of the IQ motif.

Expressed in brain liver lung kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart pancreas liver intestine and adipose tissues. Developmental Stage: Expressed in fetal tissues. More abundant in kidney.

The IQ motif which is involved in calmodulin binding overlaps with the binding domain for nuclear receptors and transcription factors. Its phosphorylation probably allows a switch between the two activities of the protein (By similarity).

Contains 1 HTH cro/C1-type DNA-binding domain. Summary: This protein is a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene. Brendel. C. . et al. . (2002) Mol. Endocrinol. 16 (6). 1367-1377.